Journal article
The "sweet" Side of the protein corona: Effects of glycosylation on nanoparticle-cell interactions
S Wan, PM Kelly, E Mahon, H Stöckmann, PM Rudd, F Caruso, KA Dawson, Y Yan, MP Monopoli
ACS Nano | Published : 2015
DOI: 10.1021/nn506060q
Abstract
The significance of a protein corona on nanoparticles in modulating particle properties and their biological interactions has been widely acknowledged. The protein corona is derived from proteins in biological fluids, many of which are glycosylated. To date, the glycans on the proteins have been largely overlooked in studies of nanoparticle-cell interactions. In this study, we demonstrate that glycosylation of the protein corona plays an important role in maintaining the colloidal stability of nanoparticles and influences nanoparticle-cell interactions. The removal of glycans from the protein corona enhances cell membrane adhesion and cell uptake of nanoparticles in comparison with the fully..
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Grants
Awarded by University of Melbourne
Funding Acknowledgements
This work was supported by the European Union's Seventh Framework Programme (EU FP7) under grant agreement no: 309329 (NANOSOLUTIONS), the EU FP7 program HighGlycan, Grant No. 278535, SFI PI Award (12/IA/1422; awarded to K.D.), the Irish Research Council under the EMBARK scheme (RS/2011/106), the Australian Research Council under the Australian Laureate Fellowship (F.C., FL120100030), the Discovery Early Career Researcher Award (Y.Y, DE130100488), the Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology (CE140100036), and the University of Melbourne under the Dyason Fellowship (Y.Y.).